Let's Meet at SLAS 2023 - Booth #428

Stop by Booth #428 to watch a demonstration of the AVENIO Edge System for automated library prep. This truly walk-away, easy-to-adopt automation system for WGS and target enrichment workflows carries out all steps of library preparation—including normalization and pooling—all right on the deck.

If you have your own liquid handling system, ask us about automated KAPA Library Prep workflows on non-Roche liquid handling systems, including complimentary method development on select liquid handlers.

We’ll also have several scientific posters to share: KAPA HyperExome probes implemented on Roche's AVENIO Edge System; and KAPA EvoPlus on two non-Roche systems—the Biomek i5 Automated Workstation (Beckman Coulter Life Sciences) and Sciclone G3 NGSx Workstation (PerkinElmer). 

What is the SLAS International Conference and Exhibition?
The Society for Laboratory Automation and Screening (SLAS) International Conference and Exhibition features scientific sessions in nine tracks delivered by a diverse group of scientists, academic researchers, and industry professionals from around the world. SLAS 2023 will provide the platform to connect, collaborate and showcase your research, products and services to an eager and energized audience. 

Location

San Diego Convention Center
111 W Harbor Dr.
San Diego, CA 92101

Booth Hours

• Sunday, Feb 26th 4:00pm - 7:00pm
• Monday, Feb 27th 10:00am - 6:00pm
• Tuesday, Feb 28th 10:00am - 6:00pm

Featured Products in Booth #428

AVENIO Edge System is designed for users at any level, and provides true walk-away NGS library prep automation—including WGS, target enrichment, pooling, and 
normalization.

Automated NGS workflows are available on a variety of liquid handlers, developed in collaboration with other vendors—so when you are ready to automate, you can plug-and-play these existing methods.

KAPA HyperPETE primer extension target enrichment offers both the performance of hybrid capture and the simplicity of amplicon workflows.

Scientific Posters

High-throughput Automated Library Preparation of KAPA EvoPlus Kit on PerkinElmer’s Sciclone G3 NGSx Liquid Handler

Authors: Lichun Li¹, Marsha McMakin¹, Rachel Kasinskas¹, Jyotsana Gupta², Nicole Madamba²

¹Roche Diagnostics/Roche Sequencing & Life Science, Wilmington, MA

²PerkinElmer, Inc., Waltham, MA 

Summary: The Roche KAPA EvoPlus Kit is a new, high-performing enzymatic fragmentation solution for DNA library preparation. KAPA EvoPlus Kit is tolerant of many common inhibitors, includes convenient automation-friendly ReadyMixes in tubes or in plates, and is stable at room temperature for 24 hours.

The KAPA EvoPlus Kit has been successfully automated on the PerkinElmer Sciclone G3 NGSx liquid handler using an automated method that is compatible with either the tubed or plated reagent formats, and can simultaneously process up to 96 samples. This automated method:

• generates libraries comparable to manual preparations, including PCR-free libraries, across a range of input amounts (10 ng, 100 ng, and 500 ng human gDNA were tested)
• produces libraries with consistent size and yield with no plate effects
• reduces the hands-on time required for library preparation.

_{Sciclone is a trademark of PerkinElmer, Inc.}

Inter-site workflow performance of a fully automated sample preparation system for NGS applications: integration with KAPA HyperExome probes for whole exome sequencing, the AVENIO Edge instrument, and navify® Mutation Caller

Authors: Yanpeng Xi,¹ Lara Sucheston-Campbell,¹ Pierre-Luc Janvier,¹ Helle Sorensen,² and Alexandra Markus¹

¹Roche Diagnostics Solutions, Pleasanton, CA
²Roche Diagnostics Solutions, Santa Clara, CA
_{Disclosures: Sponsored by Roche Sequencing Solutions (Pleasanton, CA, USA), part of the Roche organization in collaboration with Discovery Life Sciences (Huntsville, AL, USA) and Max Planck Institute (Berlin, Germany), L.S-C. is currently employed by Immunovia, Inc.}

Summary: AVENIO Edge System is a fully automated NGS sample preparation system for library preparation, target enrichment, pooling, normalization and quantification. This study evaluated the inter-site performance of the AVENIO Edge System workflow for whole-exome sequencing (WES) with the KAPA HyperExome Probes (in comparison to the manual workflow) at four sites—two independent labs (sites A and B) and two Roche sites—for a total of seven runs across five instruments at four sites.

The resulting sequencing data demonstrate that the AVENIO Edge System for NGS sample prep automation, integrated with navify® Mutation Caller for variant calling, yields:

• high inter-instrument reproducibility
• high concordance of all metrics between manual and automated methods
• robust inter-site workflow and variant detection performance compared to a manual workflow for WES using KAPA HyperExome Probes.

Biomek i5 Workstation Automation of KAPA EvoPlus Kit (96-Well Plate)

Authors: Jenni Taller¹, Zach Smith¹, Lichun Li², Marsha McMakin²

¹Beckman Coulter Life Sciences, Indianapolis, IN

²Roche Diagnostics/Roche Sequencing & Life Science, Wilmington, MA

Summary: The Roche KAPA EvoPlus Kit is an enzymatic DNA fragmentation library preparation kit designed for a wide range of sample types and inputs, and is compatible with genomic DNA as well as low-quality DNA. The new automated method described in this poster implements KAPA EvoPlus Kits on the Biomek i5 Multichannel Workstation using the pre-plated 96-well reagent format, and offers:

• simultaneous sample processing with minimal sample-to-sample contamination 
• full walk-away capability, with optional start /stop points for flexibility in workflow scheduling, processing specifications, or throughput requirements
• flexibility in sample number, incubation times, and post-ligation and post-amplification cleanup options.

This poster demonstrates successful library preparation on the Biomek i5 Multichannel Workstation using the minimum allowable DNA input of 10 ng for KAPA EvoPlus. The method can be easily implemented on other Biomek i5 Workstations and supported by the application support team, making it a turn-key solution for fast implementation.

_{Biomek is a trademark of Beckman Coulter, Inc.}

Visit https://www.eventscribe.net/2023/SLAS2023/ for full poster abstracts and poster locations.

KAPA products are for Research Use Only. Not for use in diagnostic procedures.

AVENIO Edge System is a Class 1 IVD instrument and is intended for downstream diagnostic applications.
AVENIO Edge reagents and workflows are for Research Use Only. They are not intended for diagnostic applications

AVENIO, KAPA and navify are trademarks of Roche. Other product names and trademarks are the property of their respective owners.

Biomek is a trademark of Beckman Coulter, Inc. and Sciclone is a trademark of PerkinElmer, Inc.